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Abstract: Background: The development of the amniote limb has been an important model system to study patterning mechanisms and morphogenesis. For proper growth and patterning, it requires the interaction between the distal sub-apical mesenchyme and the apical ectodermal ridge (AER) that involve the separate implementation of coordinated and tissue-specific genetic programs. Results: Here, we produce and analyze the transcriptomes of both distal limb mesenchymal progenitors and the overlying ectodermal cells, following time-coursed dissections that cover from limb bud initiation to fully patterned limbs. The comparison of transcriptomes within each layer as well as between layers over time, allowed the identification of specific transcriptional signatures for each of the developmental stages. Special attention was given to the identification of genes whose transcription dynamics suggest a previously unnoticed role in the context of limb development and also to signaling pathways enriched between layers. Conclusion: We interpret the transcriptomic data in light of the known development pattern and we conclude that a major transcriptional transition occurs in distal limb buds between E9.5 and E10.5, coincident with the switch from an early phase continuation of the signature of trunk progenitors, related to the initial proximo distal specification, to a late intrinsic phase of development.
Fuente: Dev Dyn . 2022 Sep;251(9):1550-1575
Publisher: Wiley-Liss
Year of publication: 2022
No. of pages: 26
Publication type: Article
DOI: 10.1002/dvdy.394
ISSN: 1058-8388,1097-0177
Publication Url: https://www.doi.org/10.1002/dvdy.394
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FERNANDEZ-GUERRERO, MARC
SOFIA ZDRAL NOGUERO
CASTILLA-IBEAS, ALEJANDRO
LOPEZ-DELISLE, LUCILLE
DUBOULE, DENIS
MARIA ANGELES ROS LASIERRA
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