Searching. Please wait…
1582
37
171
29406
4423
2606
347
392
Abstract: The emergence of circulating DNA analysis in blood during the past decade has responded to the need for noninvasive alternatives to classical tissue biopsies. This has coincided with the development of techniques that allow the detection of low-frequency allele variants in clinical samples that typically carry very low amounts of fragmented DNA, such as plasma or FFPE samples. Enrichment of rare variants by nuclease-assisted mutant allele enrichment with overlapping probes (NaME-PrO) enables a more sensitive detection of mutations in tissue biopsy samples alongside standard qPCR detection assays. Such sensitivity is normally achieved by other more complex PCR methods, such as TaqMan qPCR and digital droplet PCR (ddPCR). Here we describe a workflow of mutation-specific nuclease-based enrichment combined with a SYBR Green real-time quantitative PCR detection method that provides comparable results to ddPCR. Using a PIK3CA mutation as an example, this combined workflow enables detection and accurate prediction of initial variant allele fraction in samples with a low mutant allele frequency (<1%) and could be applied flexibly to detect other mutations of interest
Fuente: Methods in Molecular Biology, 2023, 2621, 41-56
Publisher: Springer Nature
Year of publication: 2023
No. of pages: 16
Publication type: Article
DOI: 10.1007/978-1-0716-2950-5_4
ISSN: 1064-3745,1940-6029
Publication Url: https://doi.org/10.1007/978-1-0716-2950-5_4
SCOPUS
Citations
Google Scholar
Metrics
Read publication
KERAITE, IEVA
VIRGINIA ALVAREZ GARCIA
LESLIE, NICHOLAS R.
Back