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Smurf1 knocked-down, mesenchymal stem cells and BMP-2 in an electrospun system for bone regeneration

Abstract: A sandwich-like system, fabricated with electrospun, poly(lactic-co-glycolic-acid) (PLGA) membranes incorporating either human recombinant bone morphogenetic protein 2 (BMP-2) enriched microspheres, rat bone marrow mesenchymal stem cells (rMSC), or rMSC with their Smurf1 (SMAD ubiquitin regulatory factor-1) expression knocked down by means of siRNA (rMSC573) at varying densities was evaluated in a rat calvarial, critical-size defect. The behavior of four membrane varieties, fabricated with different PLGA copolymers, was initially studied in rMSC cultures to decide on optimal membrane degradation and cell proliferation and differentiation characteristics. PLGA75:25 provided the most stable structure, and favored the cell environment. Radiological and histological analyses indicated bone repair in animals treated with the PLGA75:25 bioactivated systems. We found no synergist interaction between BMP-2 and rMSC 8 to 12 weeks postimplantation. By contrast, synergistic defect repair of around 85% was detected after 8 weeks of combined BMP-2 and rMSC573 treatment.

 Fuente: Biomacromolecules. 2014 Apr 14;15(4):1311-22

Editorial: American Chemical Society

 Año de publicación: 2014

Nº de páginas: 12

Tipo de publicación: Artículo de Revista

 DOI: 10.1021/bm401854d

ISSN: 1525-7797,1526-4602

Autores/as

RODRÍGUEZ EVORA, M

GARCÍA PIZARRO, E

ROSARIO, CARLOS DEL

JAVIER PEREZ LOPEZ

REYES, RICARDO

DELGADO, ARACELI