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Sputum Microbiome Dynamics in Chronic Obstructive Pulmonary Disease Patients during an Exacerbation Event and Post-Stabilization

Abstract: BACKGROUND: Chronic obstructive pulmonary disease (COPD) affects up to 65 million people worldwide, and COPD exacerbation causes tissue damage and subsequent loss of lung function. It is a multifactorial event in which respiratory infections are involved, but little is known about its dynamics. OBJECTIVES: The objective of our study was to determine the microbiome composition during an exacerbation event and post-stabilization. METHODS: We conducted an observational analytical study of a cohort of 55 COPD patients in which 2 sputum samples (the first taken during an exacerbation event and the second during clinical post-stabilization) were submitted to 16s RNA ribosomal analysis by Illumina Miseq Next Generation Sequencing (NGS). The presence of respiratory viruses was also determined. RESULTS: Our study found a stable microbiome composition in the post-stabilization sputum samples of COPD patients, and 4 additional microbiomes in samples taken during the exacerbation, 3 of which showed a marked dysbiosis by Haemophilus, Pseudomonas, and Serratia. The fourth exacerbation microbiome had a very similar composition to post-stabilization samples, but some pathogens such as Moraxella and respiratory viruses were also found. CONCLUSIONS: Our study reveals the main protagonists involved in lung microbiome dynamics during an exacerbation event and post-stabilization in COPD patients by NGS analysis.

Otras publicaciones de la misma revista o congreso con autores/as de la Universidad de Cantabria

 Fuente: Respiration. 2019;98(5):447-454

Editorial: Karger

 Año de publicación: 2019

Nº de páginas: 8

Tipo de publicación: Artículo de Revista

 DOI: 10.1159/000501988

ISSN: 0025-7931,1423-0356

Url de la publicación: https://www.doi.org/10.1159/000501988

Autores/as

JUAN CARLOS LOPEZ CARO

GARCÍA RIVERO, JUAN LUIS

VILLANUEVA, MANUEL

SAINZ, JESÚS

GONZÁLEZ ASTORQUI, PABLO

HIERRO, MARGARITA

MARIANO RODRIGUEZ PORRES

MIRA, ALEX

RODRIGUEZ, JUAN CARLOS

GALIANA, ANTONIO

ACINAR-MICROBIOME STUDY GROUP