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Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals

Abstract: There is an ongoing need of developing sensitive and specific methods for the determination of SARS-CoV-2 seroconversion. For this purpose, we have developed a multiplexed flow cytometric bead array (C19BA) that allows the identification of IgG and IgM antibodies against three immunogenic proteins simultaneously: the spike receptor-binding domain (RBD), the spike protein subunit 1 (S1) and the nucleoprotein (N). Using different cohorts of samples collected before and after the pandemic, we show that this assay is more sensitive than ELISAs performed in our laboratory. The combination of three viral antigens allows for the interrogation of full seroconversion. Importantly, we have detected N-reactive antibodies in COVID-19-negative individuals. Here we present an immunoassay that can be easily implemented and has superior potential to detect low antibody titers compared to current gold standard serology methods.

 Fuente: Communications Biology, 2021, 4(1), 486

 Editorial: Nature

 Año de publicación: 2021

 Nº de páginas: 10

 Tipo de publicación: Artículo de Revista

 DOI: 10.1038/s42003-021-02011-6

 ISSN: 2399-3642

 Proyecto español: SAF2017-87301-R

 Url de la publicación: https://doi.org/10.1038/s42003-021-02011-6

Autoría

EGIA-MENDIKUTE, LEIRE

BOSCH, ALEXANDRE

PRIETO-FERNÁNDEZ, ENDIKA

LEE, SO YOUNG

JIMÉNEZ-LASHERAS, BORJA

GARCÍA DEL RÍO, ANA

ANTOÑANA-VILDOSOLA, ASIER

BRUZZONE, CHIARA

BIZKARGUENAGA, MAIDER

EMBADE, NIEVES

GIL-REDONDO, RUBÉN

MARTÍNEZ-CHANTAR, MARÍA LUZ

ABRESCIA, NICOLA G A

MATO, JOSÉ M

MILLET, ÓSCAR

PALAZÓN, ASÍS