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Abstract: GES (Guiana Extended Spectrum) carbapenemases belong to ?minor class A carbapenemases? and its prevalence could be underestimated due to the lack of specific tests. The aim of this study was to develop an easy PCR method to differentiate between GES ?-lactamases with or without carbapenemase activity, based on an allelic discrimination system of SNPs that encode E104K and G170S mutations, without need of sequencing. Two pair of primers and Affinity Plus probes, labeled with different fluorophores; FAM/IBFQ and YAK/IBFQ, were designed for each one of the SNPs. This allelic discrimination assay allows to detect in real time the presence of all type of GES- ?-lactamases, being able to differentiate between carbapenemases and extended-spectrum ?-lactamase (ESBL), through a quick PCR test that avoid costly sequencing approaches and could help to decrease the current underdiagnosis of minor carbapenemases that scape of phenotypic screenings.
Fuente: Journal of Microbiological Methods, 2023, 207, 106694
Editorial: Elsevier
Año de publicación: 2023
Nº de páginas: 6
Tipo de publicación: Artículo de Revista
DOI: 10.1016/j.mimet.2023.106694
ISSN: 0167-7012,1872-8359
Proyecto español: PI18CIII/00030
Url de la publicación: https://doi.org/10.1016/j.mimet.2023.106694
Consultar en UCrea Leer publicación
PABLO-MARCOS, D.
SILLER, M.
AGÜERO, J.
ÁLVAREZ-JUSTEL, A.
GARCÍA-FERNÁNDEZ, S.
VELASCO DE LA FUENTE, S.
GOICOECHEA, P.
RODRÍGUEZ-LOZANO, J.
OCAMPO-SOSA, A.
LUCAS-FERNÁNDEZ, J.
MARIA DEL CARMEN FARIÑAS ALVAREZ
FERNÁNDEZ, J.
FREILE-RIBOT, P.A.
ARACIL, B.
OTEO-IGLESIAS, J.
CALVO-MONTES, J.
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