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 Detalle_Publicacion

TOR complex 1 negatively regulates NDR kinase Cbk1 to control cell separation in budding yeast

Abstract: The target of rapamycin (TOR) signalling pathway plays a key role in the coordination between cellular growth and the cell cycle machinery in eukaryotes. The underlying molecular mechanisms by which TOR might regulate events after anaphase remain unknown. We show for the first time that one of the 2 TOR complexes in budding yeast, TORC1, blocks the separation of cells following cytokinesis by phosphorylation of a member of the NDR (nuclear Dbf2-related) protein-kinase family, the protein Cbk1. We observe that TORC1 alters the phosphorylation pattern of Cbk1 and we identify a residue within Cbk1 activation loop, T574, for which a phosphomimetic substitution makes Cbk1 catalytically inactive and, indeed, reproduces TORC1 control over cell separation. In addition, we identify the exocyst component Sec3 as a key substrate of Cbk1, since Sec3 activates the SNARE complex to promote membrane fusion. TORC1 activity ultimately compromises the interaction between Sec3 and a t-SNARE component. Our data indicate that TORC1 negatively regulates cell separation in budding yeast by participating in Cbk1 phosphorylation, which in turn controls the fusion of secretory vesicles transporting hydrolase at the site of division

 Fuente: PLoS biology, 2023, 21(8), e3002263

Editorial: Public Library os Science

 Año de publicación: 2023

Nº de páginas: 41

Tipo de publicación: Artículo de Revista

 DOI: 10.1371/journal.pbio.3002263

ISSN: 1544-9173,1545-7885

Url de la publicación: https://doi.org/10.1371/journal.pbio.3002263

Autores/as

BECH-SERRA, JOAN J.

TORRE, CAROLINA DE LA

BRACE, JENNIFER L.

WEISS, ERIC L.

QUERALT, ETHEL