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 Detalle_Publicacion

Induction of proinflammatory cytokines in human lung epithelial cells during Rhodococcus equi infection.

Abstract: Rhodococcus equi is an opportunistic human pathogen associated with immunosuppressed people. While the interaction of R. equi with macrophages has been comprehensively studied, little is known about its interactions with non-phagocytic cells. Here, we characterized the entry process of this bacterium into human lung epithelial cells. The invasion is inhibited by nocodazole and wortmannin, suggesting that the phosphatidylinositol 3-kinase pathway and microtubule cytoskeleton are important for invasion. Pre-incubation of R. equi with a rabbit anti-R. equi polyclonal antiserum resulted in a dramatic reduction in invasion. Also, the invasion process as studied by immunofluorescence and scanning electron microscopy indicates that R. equi make initial contact with the microvilli of the A549 cells, and at the structural level, the entry process was observed to occur via a zipper-like mechanism. Infected lung epithelial cells upregulate the expression of cytokines IL-8 and IL-6 upon infection. The production of these pro-inflammatory cytokines was significantly enhanced in culture supernatants from cells infected with non-mucoid plasmid-less strains when compared with cells infected with mucoid strains. These results demonstrate that human airway epithelial cells produce pro-inflammatory mediators against R. equi isolates.

Otras publicaciones de la misma revista o congreso con autores/as de la Universidad de Cantabria

 Fuente: Journal of medical microbiology, 2013, 62(8), 1144-1152

Editorial: Microbiology Society

 Año de publicación: 2013

Nº de páginas: 9

Tipo de publicación: Artículo de Revista

 DOI: 10.1099/jmm.0.056234-0

ISSN: 0022-2615,1473-5644

 Proyecto español: CGL2008-04559/BOS

Url de la publicación: https://doi.org/10.1099/jmm.0.056234-0

Autoría

REMUZGO-MARTÍNEZ, SARA

LILIAN PILARES ORTEGA

ÁLVAREZ RODRÍGUEZ, LORENA

ARAZAMENDI-ZALDUNBIDE, MAITANE

DANIEL PADILLA ACEBO

JOSE RAMOS VIVAS