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Localization of acetylcholine, alpha 7-NAChR and the antimicrobial peptide piscidin 1 in the macrophages of fsh gut: evidence for a cholinergic system, diverse macrophage populations and polarization of immune responses

Abstract: The recognition and elimination of invading pathogens are vital for host survival. Macrophages play a central role in host protection and cells functionally reminiscent of vertebrate macrophages are present in all multicellular organisms. A pattern responsible for bacterial recognition found on the surface of macrophages is CD14. These cells possess a repertoire of antimicrobial molecules stored in their granules and lysosomes. Polarization states observed in mammalian macrophages termed M1 and M2 also likely exist in fish macrophages. Markers for macrophage subtypes are slowly but definitively emerging in fish species. In the present study cell markers such as CD14, acetylcholine, alpha 7 acetylcholine nicotinic receptor (nAChR) subtype, the inducible nitric oxidase synthase (iNOS), and the antimicrobial peptide piscidin 1 are reported for the first time in the intestinal macrophages of both catfish Heteropneustes fossilis (Bloch, 1794) and the African bonytongue Heterotis niloticus (Cuvier, 1829) along the anterior and the posterior axis and the concentric muscle layers. Many antimicrobial effector responses of vertebrate macrophages including respiratory burst and NO induction are similar across the diverse animal taxa. Antibodies against calbindin coupled with ones to VAChT and tubulin revealed the localization of myenteric and submucosal plexuses, which are made up of enteric neurons, glial cells, and nerves near macrophages. Current studies allow for the elucidation of multiple roles of macrophages in disease models providing an insight into their in vivo function in fish.

 Fuente: Fishes, 2023, 8(1), 43

 Editorial: MDPI

 Año de publicación: 2023

 Tipo de publicación: Artículo de Revista

 DOI: 10.3390/fishes8010043

 ISSN: 2410-3888

 Url de la publicación: https://doi.org/10.3390/fishes8010043

Autoría

ZACCONE, GIACOMO

ALESCI , ALESSIO

MOKHTAR , DOAA

ARAGONA , MARIALUISA

GUERRERA , MARIA CRISTINA

CAPILLO , GIOELE

ALBANO , MARCO

FERNANDES , JORGE

VISWANATH , KIRON

SAYED, RAMY K. A.

HUSSEIN , MARWA

LO CASCIO, PATRIZIA

KUCIEL , MICHAL

ZUWALA , KRYSTYNA

LAURIANO, EUGENIA RITA