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Monitoring bacterial conjugation by optical microscopy

Abstract: Bacterial conjugation is the main mechanism for horizontal gene transfer, conferring plasticity to the genome repertoire. This process is also the major instrument for the dissemination of antibiotic resistance genes. Hence, gathering primary information of the mechanism underlying this genetic transaction is of a capital interest. By using fluorescent protein fusions to the ATPases that power conjugation, we have been able to track the localization of these proteins in the presence and absence of recipient cells. Moreover, we have found that more than one copy of the conjugative plasmid is transferred during mating. Altogether, these findings provide new insights into the mechanism of such an important gene transfer device.

 Authorship: Carranza G., Menguiano T., Valenzuela-Gómez F., García-Cazorla Y., Cabezón E., Arechaga I.,

 Fuente: Front Microbiol . 2021 Oct 4;12:750200

 Publisher: Frontiers Research Foundation

 Year of publication: 2021

 No. of pages: 10

 Publication type: Article

 DOI: 10.3389/fmicb.2021.750200

 ISSN: 1664-302X

 Spanish project: BFU2016-78521-R

 Publication Url: https://www.doi.org/10.3389/fmicb.2021.750200

Authorship

CARRANZA, GERARDO

FERNANDO VALENZUELA GOMEZ

GARCÍA CAZORLA, YOLANDA