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Abstract: Fundamental research and drug development for personalized medicine necessitates cell cultures from defined genetic backgrounds. However, providing sufficient numbers of authentic cells from individuals poses a challenge. Here, we present a new strategy for rapid cell expansion that overcomes current limitations. Using a small gene library, we expanded primary cells from different tissues, donors, and species. Cell-type-specific regimens that allow the reproducible creation of cell lines were identified. In depth characterization of a series of endothelial and hepatocytic cell lines confirmed phenotypic stability and functionality. Applying this technology enables rapid, efficient, and reliable production of unlimited numbers of personalized cells. As such, these cell systems support mechanistic studies, epidemiological research, and tailored drug development.
Fuente: Nature Communications 2018; 9(1):994
Publisher: Nature Publishing Group
Year of publication: 2018
No. of pages: 12
Publication type: Article
DOI: 10.1038/s41467-018-03408-4
ISSN: 2041-1723
Publication Url: https://dx.doi.org/10.1038/s41467-018-03408-4
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LIPPS, CHRISTOPH
KLEIN, FRANZISKA
WAHLICHT, TOM
SEIFFERT, VIRGINIA
BUTUEVA, MILADA
ZAUERS, JEANNETTE
TRUSCHEL, THERESA
LUCKNER, MARTIN
KÖSTER, MARIO
MACLEOD, RODERICK
PEZOLDT, JÖRN
HÜHN, JOCHEN
YUAN, QINGGONG
MÜLLER, PETER PAUL
KEMPF, HENNING
ZWEIGERDT, ROBERT
DITTRICH-BREIHOLZ, OLIVER
PUFE, THOMAS
JOSE ANTONIO RIANCHO MORAL
MARIA CAROLINA SAÑUDO CAMPO
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