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Combined fluorescence and reflectance spectroscopy for in vivo quantification of cancer biomarkers in low- and high-grade glioma surgery

Abstract: Biomarkers are indicators of biological processes and hold promise for the diagnosis and treatment of disease. Gliomas represent a heterogeneous group of brain tumors with marked intra- and inter-tumor variability. The extent of surgical resection is a significant factor influencing post-surgical recurrence and prognosis. Here, we used fluorescence and reflectance spectral signatures for in vivo quantification of multiple biomarkers during glioma surgery, with fluorescence contrast provided by exogenously-induced protoporphyrin IX (PpIX) following administration of 5-aminolevulinic acid. We performed light-transport modeling to quantify multiple biomarkers indicative of tumor biological processes, including the local concentration of PpIX and associated photoproducts, total hemoglobin concentration, oxygen saturation, and optical scattering parameters.We developed a diagnostic algorithm for intra-operative tissue delineation that accounts for the combined tumor-specific predictive capabilities of these quantitative biomarkers. Tumor tissue delineation achieved accuracies of up to 94% (specificity=94%, sensitivity=94%) across a range of glioma histologies beyond current state-of-the-art optical approaches, including state-of-the-art fluorescence image guidance. This multiple biomarker strategy opens the door to optical methods for surgical guidance that use quantification of well-established neoplastic processes. Future work would seek to validate the predictive power of this proof-of-concept study in a separate larger cohort of patients.

 Autoría: Valdés P., Kim A., Leblond F., Conde O., Harris B., Paulsen K., Wilson B., Roberts D.,

 Fuente: Journal of Biomedical Optics, 2011, 16(11), 116007

 Editorial: Society of Photo-optical Instrumentation Engineers (SPIE)

 Fecha de publicación: 01/11/2011

 Nº de páginas: 14

 Tipo de publicación: Artículo de Revista

 DOI: 10.1117/1.3646916

 ISSN: 1083-3668,1560-2281

 Url de la publicación: https://doi.org/10.1117/1.3646916

Autoría

VALDÉS, PABLO A.

KIM, ANTHONY

LEBLOND, FREDERIC

HARRIS, BRENT T.

PAULSEN, KEITH D.

WILSON, BRIAN C.

ROBERTS, DAVID W.