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Estimation of antibody-verified eplet mismatch load, 2-field HLA resolution vs imputation in a large cohort of european donors

Abstract: Background The assessment of class II eplet mismatch load is useful to determine the risk of chronic rejection in solid organ transplantation. However, high-resolution (2-field) HLA typing is mandatory to accurately define eplet mismatches. The imputation of the most frequent allele has been used in retrospective studies. Here, we sought to investigate the concordant of antibody-verified (AbV) eplet load in different class II alelles between real 2-field HLA typing and HLA imputed by most frequent allele in a large White cohort. Materials and Methods The allelic frequency of the different HLA class II loci was calculated using a database of high-resolution typing of 23,628 voluntary Spanish bone marrow donors obtained from the Spanish Registry of Bone Marrow Donors, managed by the Josep Carreras Foundation. The AbV eplet count in the different class II alleles was performed using the HLA-Matchmaker v3.1 algorithm. Results The probability of imputing the correct allele compared to the most frequent for DRB1 and DQB1 loci was 69.3% and 53.0%, respectively. However, studying the less frequent alleles, the same AbV eplet load was observed in 82.22% and 76.15%, respectively. Conclusions Our data show that despite the potential error in the imputation of class II HLA typing, the number of AbV eplets is not significantly over- or underestimated in our population. Until high-resolution typing is widely established for solid organ transplantation, retrospective studies of AbV eplet mismatch load with imputed high-resolution types provide an acceptable outcome in White people.

Otras publicaciones de la misma revista o congreso con autores/as de la Universidad de Cantabria

 Fuente: Transplantation Proceedings, 2022, 54(9), 2414-2418

Editorial: Elsevier

 Año de publicación: 2022

Nº de páginas: 5

Tipo de publicación: Artículo de Revista

 DOI: 10.1016/j.transproceed.2022.09.011

ISSN: 0041-1345,1873-2623

Url de la publicación: https://doi.org/10.1016/j.transproceed.2022.09.011

Autoría

RENUNCIO-GARCÍA, MÓNICA

GONZÁLEZ LÓPEZ, ELENA

CARRERAS, ENRIC

VILLA, JULIANA

ROMÓN ALONSO, ÍÑIGO

ROA-BAUTISTA, ADRIEL

GUTIÉRREZ LARRAÑAGA, MARÍA

COMINS BOO, ALEJANDRA

IRURE-VENTURA, JUAN

SAN SEGUNDO, DAVID