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Autoinhibition of TBCB regulates EB1-mediated microtubule dynamics

Abstract: Tubulin cofactors (TBCs) participate in the folding, dimerization, and dissociation pathways of the tubulin dimer. Among them, TBCB and TBCE are two CAP-Gly domain-containing proteins that together efficiently interact with and dissociate the tubulin dimer. In the study reported here we showed that TBCB localizes at spindle and midzone microtubules during mitosis. Furthermore, the motif DEI/M-COO? present in TBCB, which is similar to the EEY/F-COO? element characteristic of EB proteins, CLIP-170, and ?-tubulin, is required for TBCE?TBCB heterodimer formation and thus for tubulin dimer dissociation. This motif is responsible for TBCB autoinhibition, and our analysis suggests that TBCB is a monomer in solution. Mutants of TBCB lacking this motif are derepressed and induce microtubule depolymerization through an interaction with EB1 associated with microtubule tips. TBCB is also able to bind to the chaperonin complex CCT containing ?-tubulin, suggesting that it could escort tubulin to facilitate its folding and dimerization, recycling or degradation.

 Fuente: Cellular and Molecular Life Sciences, 2013, 70, 357-371

 Editorial: Springer

 Fecha de publicación: 01/01/2013

 Nº de páginas: 15

 Tipo de publicación: Artículo de Revista

 DOI: 10.1007/s00018-012-1114-2

 ISSN: 1420-682X,1420-9071

 Proyecto español: BFU2007-64882

 Url de la publicación: https://doi.org/10.1007/s00018-012-1114-2

Autoría

GERARDO CARRANZA FERRER

RAQUEL CASTAÑO COBO

GONÇALVEZ, JOÃO

SOARES, HELENA

MARENCHINO, MARCO

CAMPOS-OLIVAS, RAMÓN

MONTOYA, GUILLERMO

JUAN CARLOS ZABALA OTAÑO