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Abstract: The most abundant populations of non-neoplastic cells in the glioblastoma (GBM) microenvironment are resident microglia, macrophages and infiltrating monocytes from the blood circulation. The mechanisms by which monocytes infiltrate into GBM, their fate following infiltration, and their role in GBM growth are not known. Here we tested the hypothesis that loss of the fractalkine receptor CX3CR1 in microglia and monocytes would affect gliomagenesis. Deletion of Cx3cr1 from the microenvironment resulted in increased tumor incidence and shorter survival times in glioma-bearing mice. Loss of Cx3cr1 did not affect accumulation of microglia/macrophages in peri-tumoral areas, but instead indirectly promoted the trafficking of CD11b+CD45hiCX3CR1lowLy-6ChiLy-6G-F4/80-/low circulating inflammatory monocytes into the CNS, resulting in their increased accumulation in the perivascular area. Cx3cr1-deficient microglia/macrophages and monocytes demonstrated upregulation of IL1? expression that was inversely proportional to Cx3cr1 gene dosage. The Proneural subgroup of the TCGA GBM patient dataset with high IL1? expression showed shorter survival compared to patients with low IL1?. IL1? promoted tumor growth and increased the cancer stem cell phenotype in murine and human Proneural glioma stem cells (GSCs). IL1? activated the p38 MAPK signaling pathway and expression of monocyte chemoattractant protein (MCP-1/CCL2) by tumor cells. Loss of Cx3cr1 in microglia in a monocyte-free environment had no impact on tumor growth and did not alter microglial migration. These data suggest that enhancing signaling to CX3CR1 or inhibiting IL1? signaling in intra-tumoral macrophages can be considered as potential strategies to decrease the tumor-promoting effects of monocytes in Proneural GBM.
Fuente: Oncotarget, 2015, 6(17), 15077-15094
Editorial: Impact Journals
Año de publicación: 2015
Nº de páginas: 18
Tipo de publicación: Artículo de Revista
DOI: 10.18632/oncotarget.3730
ISSN: 1949-2553
Consultar en UCrea Leer publicación
FERNG, XI
SZULZEWSKY, FRANK
YEREVANIAN, ALEXAN
CHEN, ZHIHONG
HEINZMANN, DAVID
RASMUSSEN, RIKKE DARLING
VIRGINIA ALVAREZ GARCIA
KIM, YEONGHWAN
WANG, BINGCHENG
TAMAGNO, ILARIA
ZHOU, HAO
LI, XIAOXIA
KETTENMANN, HELMUT
RANSOHOFF, RICHARD M.
HAMBARDZUMYAN, DOLORES
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