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A degenerate primer MOB typing (DPMT) method to classify gamma-proteobacterial plasmids in clinical and environmental settings

Abstract: Transmissible plasmids are responsible for the spread of genetic determinants, such as antibiotic resistance or virulence traits, causing a large ecological and epidemiological impact. Transmissible plasmids, either conjugative or mobilizable, have in common the presence of a relaxase gene. Relaxases were previously classified in six protein families according to their phylogeny. Degenerate primers hybridizing to coding sequences of conserved amino acid motifs were designed to amplify related relaxase genes from c-Proteobacterial plasmids. Specificity and sensitivity of a selected set of 19 primer pairs were first tested using a collection of 33 reference relaxases, representing the diversity of c-Proteobacterial plasmids. The validated set was then applied to the analysis of two plasmid collections obtained from clinical isolates. The relaxase screening method, which we call ??Degenerate Primer MOB Typing?? or DPMT, detected not only most known Inc/Rep groups, but also a plethora of plasmids not previously assigned to any Inc group or Rep-type.

Otras publicaciones de la misma revista o congreso con autores/as de la Universidad de Cantabria

 Autoría: Alvarado A., Garcillán-Barcia M., de la Cruz F.,

 Fuente: PLoS One, 2012, 7(7), e40438

Editorial: Public Library of Science

 Fecha de publicación: 01/07/2012

Nº de páginas: 15

Tipo de publicación: Artículo de Revista

 DOI: 10.1371/journal.pone.0040438

ISSN: 1932-6203

Proyecto español: BFU2008-00995/BMC

Proyecto europeo: info:eu-repo/grantAgreement/EC/FP7/248919/EU/Bacterial Computing with Engineered Populations/BACTOCOM/

Url de la publicación: https://doi.org/10.1371/journal.pone.0040438