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JMJD3 intrinsically disordered region links the 3D-genome structure to TGFß-dependent transcription activation

Abstract: Enhancers are key regulatory elements that govern gene expression programs in response to developmental signals. However, how multiple enhancers arrange in the 3D-space to control the activation of a specific promoter remains unclear. To address this question, we exploited our previously characterized TGF?-response model, the neural stem cells, focusing on a ~374?kb locus where enhancers abound. Our 4C-seq experiments reveal that the TGF? pathway drives the assembly of an enhancer-cluster and precise gene activation. We discover that the TGF? pathway coactivator JMJD3 is essential to maintain these structures. Using live-cell imaging techniques, we demonstrate that an intrinsically disordered region contained in JMJD3 is involved in the formation of phase-separated biomolecular condensates, which are found in the enhancer-cluster. Overall, in this work we uncover novel functions for the coactivator JMJD3, and we shed light on the relationships between the 3D-conformation of the chromatin and the TGF?-driven response during mammalian neurogenesis.

Otras publicaciones de la misma revista o congreso con autores/as de la Universidad de Cantabria

 Autoría: Vicioso-Mantis M., Fueyo R., Navarro C., Cruz-Molina S., van Ijcken W.F.J., Rebollo E., Rada-Iglesias Á., Martínez-Balbás M.A.,

 Fuente: Nature Communications, 2022, 13, 3263

Editorial: Springer Nature

 Año de publicación: 2022

Tipo de publicación: Artículo de Revista

 DOI: 10.1038/s41467-022-30614-y

ISSN: 2041-1723

 Proyecto español: BFU2015-69248-P

Url de la publicación: https://doi.org/10.1038/s41467-022-30614-y

Autoría

VICIOSO MANTIS, MARTA

FUEYO, RAQUEL

NAVARRO, CLAUDIA

CRUZ MOLINA, SARA DE LA

IJCKEN, WILFRED F. J. VAN

REBOLLO, ELENA

MARTÍNEZ-BALBÁS, MARIÁN A.