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Toll-like receptor 4 gene polymorphisms in polymyalgia rheumatica and elderly-onset rheumatoid arthritis.

Abstract: Objectives: Coding variants in TLR4 gene have been reported to be associated with inflammatory diseases. The aim of this study was to determine whether two of these polymorphisms (Asp299Gly and Thr399Ile) of TLR4 contribute to the genetic background of polymyalgia rheumatica (PMR) and elderly-onset rheumatoid arthritis (EORA). Furthermore, we have attempted to correlate the functional consequences of these polymorphisms. Methods: 164 patients with PMR, 93 with EORA and 126 unrelated age-matched controls were genotyped. The TLR4 genotypes were determined using allele-specific primers and restriction fragment length polymorphism analysis. Association of genotypes and alleles with disease susceptibility and disease phenotypes were studied. TLR4 expression was assessed on PBMCs by flow cytometry and TLR4 function was assessed by stimulating PBMCs in vitro with LPS. Results: No significant difference in allele frequency or genotype between patients with elderly-onset inflammatory conditions and controls was observed. The Thr399Ile CC genotype was associated with a higher cumulative dose of corticosteroids in patients with PMR (p=0.031). We found no association with TLR4 expression on B cells, T cells or monocytes or a distinct phenotype of TLR4 response with the Asp299Gly or Thr399Ile genotypes. Conclusions: These results do not support the association of these TLR4 variants with two age-related inflammatory conditions. The value of determining Thr399Ile genotypes for disease prognosis in PMR should be confirmed in different populations.

 Fuente: Clinical and Experimental Rheumatology, 2011, 29(5), 795-800

 Editorial: Clinical and Experimental Rheumatology

 Año de publicación: 2011

 Nº de páginas: 6

 Tipo de publicación: Artículo de Revista

 ISSN: 0392-856X,1593-098X

 Url de la publicación: https://www.clinexprheumatol.org/abstract.asp?a=4700

Autoría

ÁLVAREZ RODRÍGUEZ, LORENA

BEARES, IGNACIO

MATA, CRISTINA

MARIA TERESA GARCIA UNZUETA

JAIME CALVO ALEN

AURRECOECHE, ELENA