Inicio de sesión


Dr. Gabriel Moncalián

Phone:(+34) 942 201934 / Fax : (+34) 942 266399


Mailing address: IBBTEC. C/ Albert Einstein 22, PCTCAN, 39011 Santander

Protein Design and Engineering


Principal Investigator

Dr. Gabriel Moncalián, Associate Profesor

Other personnel

  • Raúl Ruiz González, postdoctoral 
  • Lorena González Montes, predoctoral
  • Aurora Martín, predoctoral

Research lines

  • Structure, function and modification of polyunsaturated fatty acid synthases.
  • Optimization of the microbial production of added value fatty acids and triglycerides.
  • Optimization of proteins involved in bacterial conjugation for biotechnological applications.

Services offered

  • Design and optimization of proteins with biotechnological, biomedical or industrial interest.
  • Structural analysis of proteins by X-ray crystallography and in silico molecular threading.


  • Plasmid Offensive: Protein engineering operations (BFU2014-55534-C2-2-P)
  • Probióticos guardianes para la lucha contra la resistencia a antibióticos mediante sistemas CRISPR conjugativos (BFU2014-62007-EXP)
  • Optimización de la purificación de la catalasa obtenida de hígado de vaca (TF16-XX-007 –Laboratorios Arroyo S.L.- SODERCAN/FEDER)
  • Desarrollo de tecnología amti para el cultivo de algas en plantas de acuicultura convencionales. La biotecnología como instrumento para una acuicultura rentable y circular (Investalga S.L.).

Relevant publications (last 5 years)

  • Santín, O. and Moncalián, G. (2018). Loading of malonyl-CoA onto tandem acyl carrier protein domains of polyunsaturated fatty acid synthases. J Biol Chem. doi: 10.1074/jbc.RA118.002443. [PubMed]
  • Giner-Robles, L.; Lázaro, B.; de la Cruz, F. and Moncalián, G. (2018). fabH deletion increases DHA production in Escherichia coli expressing Pfa genes. Microbial Cell Factories. 17,88 doi: 10.1186/s12934-018-0928-5. [PubMed]

  • Zechner, E.L.; Moncalián, G. and de la Cruz, F. (2017). Relaxosomes and plasmid transfer in Gram-negative bacteria. Current Topics in Microbiology and Immunology. 413:93-113. doi: 10.1007/978-3-319-75241-9_4. [PubMed]

  • Lázaro, B.; Villa, J.A.; Santín, O.; Cabezas, M.; Milagre, C.D.F.; de la Cruz, F. and Moncalián, G. (2017). Heterologous Expression of a Thermophilic Diacylglycerol Acyltransferase Triggers Triglyceride Accumulation in Escherichia coli. Plos One. 12, e0176520. [PubMed]
  • Juarez A, Villa JA, Lanza VF, Lázaro B, de la Cruz F, Alvarez HM and Moncalián G. (2017). Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1. Microbial Cell Factories 16,35. doi: 10.1186/s12934-017-0651-7.  [PubMed]
  • Sagredo S, de la Cruz F and Moncalián G. (2016). Design of Novel Relaxase Substrates Based on Rolling Circle Replicases for Bioconjugation to DNA Nanostructures. PLoS One;11:e0152666. doi:0.1371/journal.pone.0152666. [PubMed]
  • Sagredo,S., Pirzer,T., Rafat, A.A., Goetzfried,M.A., Moncalian, G., Simmel, F.C. and de la Cruz, F. (2016).  Orthogonal protein assembly on DNA nanostructures using relaxases. Angewandte Chemie Int. Ed. 55, 4348-52. doi: 10.1002/anie.201510313. [PubMed] 
  • Fernandez Lopez R, De Toro Hernando M, Moncalian G, Garcillán-Barcia MP and de la Cruz F (2016). Comparative genomics of the conjugation region of F-like plasmids: five shades of F. Front Mol Biosci. 2016 Nov 10;3:71. eCollection 2016. doi: 10.3389/fmolb.2016.00071. [PubMed]
  • Godziszewska J, Moncalián G, Cabezas M, Bartosik AA, de la Cruz F and Jagura-Burdzy G. (2016). Concerted action of NIC relaxase and auxiliary protein MobC in RA3 plasmid conjugation. Mol Microbiol. doi: 10.1111/mmi.13401 [PubMed]
  • Wan, X.; Peng, Y-F.; Zhou, X-R.; Gong, Y-M.; Huang, F-H. and Moncalian, G. (2016). Effect of cerulenin on fatty acid composition and gene expression pattern of DHA-producing strain Colwellia psychrerythraea strain 34H. Microbial Cell Factories  15, 30. doi: 10.1186/s12934-016-0431-9. [PubMed]
  • Herrero, M.O, Moncalian, G. and Alvarez, H.M.(2016). Physiological and genetic differences among Rhodococcus species for using glycerol as a source for growth and triacylglycerol production. Microbiology 162, 384-97. doi: 10.1099/mic.0.000232. [PubMed]
  • Fernandez-López R, Ruiz R, de la Cruz F, Moncalián G. (2015). Transcription factor-based biosensors enlightened by the analyte. Front Microbiol. 6:648. doi: 10.3389/fmicb.2015.00648. [PubMed]
  • Rueda, C; Calvo, PA; Moncalian, G ; Ruiz, G and Coz, A (2015) Biorefinery options to valorize the spent liquor from sulfite pulping. J. Chem. Technol. Biotechnol.90: 2218-26. doi: 10.1002/jctb.4536.  [PubMed]
  • Carballeira JD, González-Pérez B, Moncalián G* and de la Cruz F*. (2014). A high security double lock and key mechanism in HUH relaxases controls oriT-processing for plasmid conjugation. Nucleic Acids Res. 42, 10632-43. [PubMed].
  • Villa, J.A., Cabezas, M., de la Cruz, F. and Moncalián, G. (2014). Identification of folding domains and sequence motifs critical for WS/DGAT acyltransferase activity, probed by limited proteolysis and mutagenesis. Appl Environ Microbiol. 80, 1132-41. [pubmed]
  • Francia MV, Clewell DB, de la Cruz F and Moncalián G. (2013). Catalytic domain of plasmid pAD1 relaxase TraX defines a group of relaxases related to restriction endonucleases. Proc Natl Acad Sci U S A. 110, 13606-11 [PubMed]
  • Chandler M, de la Cruz F, Dyda F, Hickman AB, Moncalian G and Ton-Hoang B. (2013). Breaking and joining single-stranded DNA: the HUH endonuclease superfamilyNat Rev Microbiol. 11, 525-38. doi: 10.1038/nrmicro3067. [PubMed]